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Document 2940
DOCN M94A2940
TI Studies on HIV infected individuals who are long term non-progressors.
Multicenter AIDS Cohort Study (MACS).
DT 9412
AU Graziosi C; Demarest JF; Vaccarezza M; Cohen OJ; Pantaleo G; Fauci AS;
NIAID, National Institutes of Health, Bethesda, MD 20892.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):19 (abstract no. 049B). Unique
Identifier : AIDSLINE ICA10/94369635
AB OBJECTIVE: To analyze viral burden and viral replication in peripheral
blood (PB) and lymph node (LN) from HIV infected individuals who are
long term non-progressors (LTNP). METHODS: Viral burden (i.e. frequency
of HIV infected cells) and viral replication were assessed by
semiquantitative DNA and RNA polymerase chain reaction (PCR) assays.
Virus distribution in lymph node and tissue architecture was determined
by in situ hybridization in combination with immunoperoxidase. RESULTS:
Eight LTNP (i.e. infected for more than 7 years, CD4+ cell slope > or =
0, no antiretroviral therapy) were studied. Viral burden and viral
replication were significantly lower, (at least 1 log), in both
mononuclear cells isolated from PB and LN of LTNP compared to the levels
observed in PB and LN of 20 normal progressors. Trapping of virus in the
follicular dendritic cell network was observed in six of eight cases. In
one case, only individual cells expressing HIV were detected, and one
case was negative for both cell-associated and extracellular (i.e.
trapped) virus. The degree of follicular hyperplasia was less in LN from
LTNP compared to progressors and no sign of tissue involution were
observed. Virus isolated from PB and LN of LTNP is infectious and
replication competent. DISCUSSION AND CONCLUSIONS: Lymphoid tissue
architecture is preserved in LTNP. Viral burden and viral replication
are markedly decreased; however, the virus that is present is
replication competent and infectious.
DE Human HIV/GROWTH & DEVELOPMENT/*ISOLATION & PURIF/PATHOGENICITY HIV
Infections/*MICROBIOLOGY Immunoenzyme Techniques In Situ Hybridization
Polymerase Chain Reaction *Virus Replication MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).